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Veterinary Pathology, Vol 25, Issue 3 193-198, Copyright © 1988 by American College of Veterinary Pathologists

ARTICLES

Double labeling immunohistological study of African swine fever virus-infected spleen and lymph nodes

I. Minguez, A. Rueda, J. Dominguez and J. M. Sanchez-Vizcaino
Instituto Nacional de Investigaciones Agrarias, Departamento de Virologia Animal, Madrid, Spain.

To identify cells in situ in which African swine fever (ASF) virus is present, a double immunohistological labeling technique was used on sections of ASF-infected spleen and lymph nodes. Cells were identified by an indirect immunoalkaline phosphatase technique using monoclonal antibodies (MoAb) reactive against different leukocyte subsets. ASF virus, detected by a direct immunoperoxidase method using swine immunoglobulin G (IgG) anti-ASF virus antigens, was not present in T helper or in T cytotoxic/suppressor lymphocytes, whereas it was detected in tissue macrophages that reacted with different MoAb (74-22-15, C4, A7, and F2). A large number of cells strongly reactive with MoAb 74-12-4 (T helper lymphocytes) were found in the marginal zone in infected spleen. In infected lymph nodes, these intensely stained cells were found in small numbers. Cells reactive with MoAb 76-2-11 (T cytotoxic/suppressor lymphocytes) were less stained in infected spleen and lymph nodes than in non-infected organs.


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M. Gómez del Moral, E. Ortuño, P. Fernández-Zapatero, F. Alonso, C. Alonso, A. Ezquerra, and J. Domínguez
African Swine Fever Virus Infection Induces Tumor Necrosis Factor Alpha Production: Implications in Pathogenesis
J. Virol., March 1, 1999; 73(3): 2173 - 2180.
[Abstract] [Full Text]


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