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Veterinary Pathology, Vol 36, Issue 5 368-378, Copyright © 1999 by American College of Veterinary Pathologists

ARTICLES

Ultrastructure of porcine circovirus in persistently infected PK-15 cells

G. W. Stevenson, M. Kiupel, S. K. Mittal and C. L. Kanitz
Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907-1175, USA. greg@addl.purdue.edu

The ultrastructure of porcine circovirus was examined in persistently infected porcine kidney (PK)-15 cells. Virus-infected PK-15 cells had large numbers of intracytoplasmic inclusions, and a few cells had intranuclear inclusions. Intracytoplasmic inclusions were dispersed throughout the cytoplasm but were most numerous in the perinuclear cytoplasm. Inclusion were of various sizes, round to oval, and electron dense and were of two general types. Inclusions of the first type were small (0.1-0.5 microm diameter), not surrounded by trilaminar membranes, and granular with indistinct margins that blended with surrounding cytoplasm. Some contained 12+/-2-nm-diameter icosahedral virions in loose aggregates or rarely forming paracrystalline arrays. Small inclusions could be sites of viral assembly or maturation. Intracytoplasmic inclusions of the second type were larger (0.5-5.0 microm diameter) and more numerous and had abrupt margins surrounded by trilaminar membranes. They were more electron dense than small inclusions and were heterogeneous, containing various proportions of aggregated virions, electron-dense crystalline lamellae of 5 nm periodicity, and/or whorls of myelinoid membranes. Virions usually formed paracrystalline arrays and occasionally were loosely aggregated. Larger inclusions were typical of autophagolysosomes. Intranuclear inclusions were not membrane bound and were often associated with reticulated nucleoli or aggregates of heterochromatin. Some inclusions were irregularly shaped aggregates of indistinct, circular 10-12-nm-diameter viruslike particles. Others were 0.1-1.0 microm in diameter, round or ring shaped, dense, and finely granular, with sharply demarcated margins.


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 Vet PatholHome page
M. Kiupel, G. W. Stevenson, J. Choi, K. S. Latimer, C. L. Kanitz, and S. K. Mittal
Viral Replication and Lesions in BALB/c Mice Experimentally Inoculated with Porcine Circovirus Isolated from a Pig with Postweaning Multisystemic Wasting Disease
Vet. Pathol., January 1, 2001; 38(1): 74 - 82.
[Abstract] [Full Text] [PDF]

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 J. Clin. Microbiol.Home page
Q. Liu, L. Wang, P. Willson, and L. A. Babiuk
Quantitative, Competitive PCR Analysis of Porcine Circovirus DNA in Serum from Pigs with Postweaning Multisystemic Wasting Syndrome
J. Clin. Microbiol., September 1, 2000; 38(9): 3474 - 3477.
[Abstract] [Full Text]


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Copyright © 1999 by the American College of Veterinary Pathologists.