This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Del Piero, F. Search for Related Content PubMed PubMed Citation Articles by Del Piero, F.

Diagnosis of Equine Arteritis Virus Infection in Two Horses by Using Monoclonal Antibody Immunoperoxidase Histochemistry on Skin Biopsies

Abstract

Two 5-year-old grade male horses presented with epiphora, rhinorrhea, conjunctival and nasal mucosal hyperemia, and dorsal and thoracic macropapular rash. Skin biopsies were collected from the affected areas, and serial sections were evaluated following hematoxylin and eosin and immunoperoxidase histochemistry staining by using a murine monoclonal antibody of the immunoglobulin G2A isotype recognizing the 30-kDa membrane protein of equine arteritis virus (EAV). In both horses, lesions consisted of mild to moderate diffuse superficial dermal edema and vasculitis with mild perivascular lymphocytic infiltrates, occasional endothelial hypertrophy, and single-cell necrosis of tunica media myocytes. Immunohistochemically, a few endothelial cells, myocytes, and pericytes containing intracytoplasmic EAV antigen were identified. Immunoperoxidase histochemistry of skin biopsies can be used as an ancillary test for the clinical diagnosis of equine viral arteritis in horses, especially when a cutaneous macropapular rash is evident.

Key words: Equine arteritis virus; immunoperoxidase histochemistry; skin biopsy; vasculitis.

This article has been cited by other articles:

				C. Bukovsky, F. Schmoll, S. Revilla-Fernandez, and H. Weissenbock Studies on the aetiology of non-suppurative encephalitis in pigs Vet Rec., October 20, 2007; 161(16): 552 - 558. [Abstract] [Full Text] [PDF]