This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martín de las Mulas, J. Articles by Jover, A. Search for Related Content PubMed PubMed Citation Articles by Martín de las Mulas, J. Articles by Jover, A.

Immunohistochemical Expression of Estrogen Receptor ß in Normal and Tumoral Canine Mammary Glands

Abstract

To date, two isoforms of estrogen receptors (ER) have been identified, cloned, and characterized from several species, estrogen receptor– (ER) and estrogen receptor–ß (ERß). Although the presence of ER has been demonstrated in normal and tumoral canine mammary tissues, the issue of ERß expression has not been addressed in the dog. In this study, we have analyzed the expression of ERß in formalin-fixed, paraffin-embedded tissue samples of nonaltered mammary gland, 30 malignant (six complex carcinoma, 12 simple carcinoma, three carcinosarcoma, and nine carcinoma or sarcoma in benign tumor), and five benign (one fibroadenoma, one complex papilloma, one complex adenoma, and two benign mixed tumors) mammary tumors of the dog by using a polyclonal ERß antibody and the avidin–biotin–peroxidase complex immunohistochemical technique. Our results show that high numbers of normal ductal and acinar epithelium and approximately one third of canine mammary tumors express ERß. This expression was higher in benign than in malignant tumors. Furthermore, expression was higher in complex and mixed histologic subtypes of malignant tumors when compared with simple subtypes.

Key words: Dogs; estrogen receptor ß; mammary gland; tumor.

Request reprints from Dr. J. Martín de las Mulas, Dpto. de A. y Anatomía Patológica Comparadas, Universidad de Córdoba, Edificio de Sanidad Animal, Campus de Rabanales, Carretera de Madrid-Cádiz, Km. 396, Córdoba 14014 (Spain). anlmagoj{at}uco.es.

This article has been cited by other articles:

				D. S. Bangari and G. W. Stevenson Carcinoma in a mixed mammary tumor in a llama (Lama glama) J Vet Diagn Invest, July 1, 2007; 19(4): 450 - 453. [Abstract] [Full Text] [PDF]

				J. Martin de las Mulas, Y. Millan, and R. Dios A Prospective Analysis of Immunohistochemically Determined Estrogen Receptor {alpha}and Progesterone Receptor Expression and Host and Tumor Factors as Predictors of Disease-free Period in Mammary Tumors of the Dog Vet. Pathol., February 1, 2005; 42(2): 200 - 212. [Abstract] [Full Text] [PDF]