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Acute Bacterial Cystitis Does not Cause Deoxyribonucleic Acid Damage Detectable by the Alkaline Comet Assay in Urothelial Cells of Dogs

Abstract

Considering the high incidence of dogs with acute bacterial cystitis (BC) and the relationship among inflammation, genotoxicity, and carcinogenesis, we conducted a case-control study comparing the frequency of deoxyribonucleic acid (DNA) lesions assessed by the comet assay between disease-free animals (13 males and 13 females) and cytology-confirmed cases of acute BC (12 males and 12 females), which was mainly caused by Staphylococcus sp. (40%) and Escherichia coli (35%). The results show no increase in DNA damage in cells obtained by bladder washings and no influence of age, sex, and breed due to acute BC. In conclusion, DNA damage was seemingly not associated with the infection by specific bacteria.

Key words: Acute bacterial cystitis; comet assay; DNA damage; dogs; urothelial cells.

Request reprints from Dr. N. S. Rocha, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia-UNESP, 18618-000 Botucatu, SP (Brazil). rochanoeme{at}fmvz.unesp.br.