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Vet Pathol 44:80-83 (2007)
© 2007 American College of Veterinary Pathologists

An Immunohistochemical and Polymerase Chain Reaction Evaluation of Feline Plasmacytic Pododermatitis

S. V. Bettenay, M. R. Lappin and R. S. Mueller

Tierdermatologie Oberhaching, Oberhaching, Germany (SVB), Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO (MRL), Medizinische Kleintierklinik, Veterinary Faculty, University of Munich, München, Germany (RSM)

Sections of 14 skin biopsies of cats with plasmacytic pododermatitis and a clinical follow-up of 12–36 months were stained with a polyclonal anti-Mycobacterium bovis (Bacille Calmette-Guerin = BCG) antibody cross-reactive to a broad spectrum of fungi and bacteria. All sections were negative for organisms within the actual footpad tissue with the anti-BCG antibody stains. Polymerase chain reaction (PCR) assays that amplify the DNA of Bartonella spp., Ehrlichia spp., Anaplasma phagocytophilum, Chlamydophila felis, Mycoplasma spp., Toxoplasma gondii, and feline herpesvirus 1 (FHV-1) were applied to tissue digests. DNA of those pathogens assessed was not amplified from tissue.

Key words: Anaplasma; anti-BCG; Bartonella; Ehrlichia; feline; feline herpesvirus 1; PCR; plasmacytic pododermatitis; Mycoplasma; Toxoplasma.

Request reprints from Dr. Ralf Mueller, Medizinische Kleintierklinik, Veterinary Faculty, University of Munich, Veterinärstrasse 13, 80539 München, Germany. E-mail: Ralf.Mueller{at}med.vetmed.uni-muenchen.de






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Copyright © 2007 by the American College of Veterinary Pathologists.