Vet Pathol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Kuwamura, M.
Right arrow Articles by Serikawa, T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuwamura, M.
Right arrow Articles by Serikawa, T.
Vet Pathol 45:586-591 (2008)
© 2008 American College of Veterinary Pathologists
ANIMAL MODELS OF HUMAN DISEASE

Pancreatic Metaplasia in the Gastro-achlorhydria in WTC-dfk Rat, a Potassium Channel Kcnq1 Mutant

M. Kuwamura, R. Okajima, J. Yamate, T. Kotani, T. Kuramoto and T. Serikawa

Laboratory of Veterinary Pathology, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan, Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan

Abstract

The WTC–deafness Kyoto (dfk) rat is a new mutant characterized by deafness and abnormal, imbalanced behavior. WTC-dfk rats carry an intragenic deletion at the Kcnq1 gene; KCNQ1 plays an important role in K+ homeostasis, and the mutation of Kcnq1 causes a cardiac long QT syndrome in humans. Here, we studied stomach lesions in these WTC-dfk rats. The most characteristic pathologic feature in the stomach was the appearance of hypertrophic gastric glands in the stomach body. The hypertrophic cells had many eosinophilic granules in their cytoplasm, and these granules were stained red with Azan stain; stained positively for trypsinogen, amylase, and chymotrypsin; and did not stain positively for pepsinogen when using immunohistochemical analysis. These staining results suggested a metaplasia toward a pancreatic acinar cells. Extensive fibrosis was found in the bottom part of the mucosa of 34-week-old WTC-dfk rats, suggesting a progression of stomach lesions with aging. Although cells that were positive for proliferating cell nuclear antigen were restricted in the area of the glandular neck in WTC control rats, positive cells in WTC-dfk rats were scattered throughout the mucosa. The parietal cells in WTC-dfk rats were negative for KCNQ1 immunohistochemical analysis. These findings indicate that a deficiency in rat Kcnq1 provokes an abnormal proliferation and differentiation of gastric glandular cells.

Key words: Eosinophilic granules; hypertrophic cell; pancreatic acinar cell; pancreatic metaplasia; rat.

Request reprints from M Kuwamura, Laboratory of Veterinary Pathology, Osaka Prefecture University, Sakai, Osaka 599-8531 (Japan). E-mail: kuwamura{at}vet.osakafu-u.ac.jp






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the American College of Veterinary Pathologists.